Gt(ROSA)26Sor^{em4(CAG-MUC15,-tdTomato)Bcgen}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em4(CAG-MUC15,-tdTomato)Bcgen}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Biocytogen LLC
• MGI ID: MGI:7620070
• Synonyms: MUC15-TG, ROSA26^Muc15
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em4(CAG-MUC15,-tdTomato)Bcgen} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em4(CAG-MUC15,-tdTomato)Bcgen} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MUC15 (143662)

  Muc15

  (MGI:2442110)

• Gt(ROSA)26Sor^{em4(CAG-MUC15,-tdTomato)Bcgen} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: CRISPR/Cas9 technology inserted a CAG promoter, loxP-flanked Stop cassette (LSL), human MUC15 coding sequence, P2A, tdTomato and WPRE into the locus. This creates a conditional-ready allele that will express the human gene only after cre-mediated excision of the STOP cassette. (J:321793)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1042 strains or lines available

References

• Original: J:321793 Han T, et al., Downregulation of MUC15 by miR-183-5p.1 promotes liver tumor-initiating cells properties and tumorigenesis via regulating c-MET/PI3K/AKT/SOX2 axis. Cell Death Dis. 2022 Mar 2;13(3):200
• All: 1 reference(s)