Gt(ROSA)26Sor^{tm4(Pik3ca*E545K*H1047R)Egan}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(Pik3ca*E545K*H1047R)Egan}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Sean E Egan
• MGI ID: MGI:7618564
• Synonyms: Rosa26-LSL-Pik3ca^E545K-H1047R
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(Pik3ca*E545K*H1047R)Egan} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:311172
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The following sequences were inserted into intron 1: splice acceptor site, a STOP cassette (loxP site flanked neomycin resistance gene cassette), Pik3ca cDNA sequence where glutamic acid codon 545 (GAA) was changed to lysine (p.E545K) and histidine codon 1047 (CAT) to arginine (p.H1047R), and bovine GH poly(A) signal sequences. (J:311172)

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm4(Pik3ca*E545K*H1047R)Egan}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:311172 Schachter NF, et al., Single allele loss-of-function mutations select and sculpt conditional cooperative networks in breast cancer. Nat Commun. 2021 Sep 2;12(1):5238
• All: 1 reference(s)