Rbck1^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Rbck1^{tm1d(EUCOMM)Hmgu}
• Name: RanBP-type and C3HC4-type zinc finger containing 1; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7618371
• Synonyms: Rbck1^-
• Gene: Rbck1 Location: Chr2:152158254-152174573 bp, - strand Genetic Position: Chr2, 74.83 cM
• Alliance: Rbck1^{tm1d(EUCOMM)Hmgu} page
• IMPC: Rbck1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:346641
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 152166003 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 152166932. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:346641)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rbck1 Mutation: 43 strains or lines available

References

• Original: J:346641 Hartley VL, et al., HOIL1 Regulates Group 3 Innate Lymphoid Cells in the Colon and Protects against Systemic Dissemination, Colonic Ulceration, and Lethality from Citrobacter rodentium Infection. J Immunol. 2023 Dec 15;211(12):1823-1834
• All: 1 reference(s)