Smarcb1^tm2.1Sho
Targeted Allele Detail

Summary

• Symbol: Smarcb1^tm2.1Sho
• Name: SWI/SNF related BAF chromatin remodeling complex subunit B1; targeted mutation 2.1, Stuart Orkin
• MGI ID: MGI:7616813
• Gene: Smarcb1 Location: Chr10:75732603-75757448 bp, - strand Genetic Position: Chr10, 38.61 cM
• Alliance: Smarcb1^tm2.1Sho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:316818
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP-flanked neomycin selection cassette was inserted into intron 1 and a single loxP site in the same orientation was inserted 0.8 kb upstream of exon 1. The neomycin selection cassette was removed by transient Cre expression in ES cells, leaving loxP sites flanking exon 1 in the final allele. Cre-mediated recombination deleted exon 1 and flanking sequences. (J:316818)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smarcb1 Mutation: 23 strains or lines available

References

• Original: J:316818 Ito T, et al., SWI/SNF chromatin remodeling complex is required for initiation of sex-dependent differentiation in mouse germline. Sci Rep. 2021 Dec 15;11(1):24074
• All: 1 reference(s)