Btk^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Btk^{tm1b(EUCOMM)Hmgu}
• Name: Bruton agammaglobulinemia tyrosine kinase; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7616771
• Synonyms: BTK^-
• Gene: Btk Location: ChrX:133443085-133484319 bp, - strand Genetic Position: ChrX, 56.18 cM
• Alliance: Btk^{tm1b(EUCOMM)Hmgu} page
• IMPC: Btk gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:346528
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 133459215 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 133460544. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:346528)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Btk Mutation: 20 strains or lines available

References

• Original: J:346528 Zhao J, et al., Bruton's tyrosine kinase regulates macrophageinduced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021 Sep;48(3)
• All: 1 reference(s)