Cdkn2a^tm4Nesh
Targeted Allele Detail

Summary

• Symbol: Cdkn2a^tm4Nesh
• Name: cyclin dependent kinase inhibitor 2A; targeted mutation 4, Norman E Sharpless
• MGI ID: MGI:7615147
• Synonyms: p16^tdTOM
• Gene: Cdkn2a Location: Chr4:89192710-89212856 bp, - strand Genetic Position: Chr4, 42.15 cM, cytoband C3-C6
• Alliance: Cdkn2a^tm4Nesh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:272509
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A DNA construct encoding a fluorescent reporter protein (tdTomato) was inserted into the endogenous first exon (exon 1alpha) of the p16/INK4apart of the gene through homologous recombination. The insertion knocks out expression of the p16/INK4a transcript and does not affect expression of the Arf transcript. A stop codon and poly-A signal were included at the end of the inserted reporter gene. An FRT-flanked neomycin selection cassette was also inserted into the first intron, and is retained in this allele. (J:272509)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdkn2a Mutation: 62 strains or lines available

References

• Original: J:272509 Liu JY, et al., Cells exhibiting strong p16 (INK4a) promoter activation in vivo display features of senescence. Proc Natl Acad Sci U S A. 2019 Feb 12;116(7):2603-2611
• All: 5 reference(s)