Tbc1d15^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tbc1d15^{tm1c(KOMP)Wtsi}
• Name: TBC1 domain family, member 15; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7612195
• Synonyms: Tbc1d15^Flox
• Gene: Tbc1d15 Location: Chr10:115033777-115087372 bp, - strand Genetic Position: Chr10, 63.51 cM
• Alliance: Tbc1d15^{tm1c(KOMP)Wtsi} page
• IMPC: Tbc1d15 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:293238
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 115054312 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 115056898. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:293238)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbc1d15 Mutation: 104 strains or lines available

References

• Original: J:293238 Choi HY, et al., p53 destabilizing protein skews asymmetric division and enhances NOTCH activation to direct self-renewal of TICs. Nat Commun. 2020 Jun 17;11(1):3084
• All: 2 reference(s)