Col1a1^{tm1(tetO-PHGDH)Mgvh}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-PHGDH)Mgvh}
• Name: collagen, type I, alpha 1; targeted mutation 1, Matthew G Vander Heiden
• MGI ID: MGI:7610185
• Synonyms: PHGDH^tetO
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-PHGDH)Mgvh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:285109
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, RMCE-ready)
• Mutation: Insertion
• Col1a1^{tm1(tetO-PHGDH)Mgvh} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PHGDH (26227)

  Phgdh

  (MGI:1355330)

• Mutation details: The targeting vector was designed to insert a human PHGDH cDNA placed downstream of a tetracycline-responsive element (TRE) promoter, and targeted via recombinase-mediated cassette exchange (RMCE) into the ubiquitously expressed collagen, type I, alpha 1 (Col1a1) gene. The targeting vector also contained a hygromycin resistance cassette. (J:285109)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:285109 Mattaini KR, et al., Increased PHGDH expression promotes aberrant melanin accumulation. BMC Cancer. 2019 Jul 22;19(1):723
• All: 3 reference(s)