Pax2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pax2^{tm1c(KOMP)Wtsi}
• Name: paired box 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7607019
• Gene: Pax2 Location: Chr19:44735057-44826310 bp, + strand Genetic Position: Chr19, 38.09 cM
• Alliance: Pax2^{tm1c(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0847_2_B03
• Germline Transmission: Earliest citation of germline transmission: J:345452
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 44748876 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 44750994. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:345452)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax2 Mutation: 44 strains or lines available

References

• Original: J:345452 Laszczyk AM, et al., Pax2 and Pax8 Proteins Regulate Urea Transporters and Aquaporins to Control Urine Concentration in the Adult Kidney. J Am Soc Nephrol. 2020;31(6):1212-1225
• All: 1 reference(s)