Tg(Slc1a3-Brainbow1.1M)1Ean
Transgene Detail

Summary

• Symbol: Tg(Slc1a3-Brainbow1.1M)1Ean
• Name: transgene insertion 1, Eva Anton
• MGI ID: MGI:7607017
• Transgene: Tg(Slc1a3-Brainbow1.1M)1Ean Location: unknown
• Alliance: Tg(Slc1a3-Brainbow1.1M)1Ean page

Transgene origin

• Strain of Origin: CBA x C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Slc1a3-Brainbow1.1M)1Ean expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc1a3 (MGI:99917)

• Mutation details: The RG-Brainbow mice incorporate a Brainbow1.1M construct under the control of a Slc1a3 (GLAST1) promoter. The Brainbow 1.1 construct has four fluorescent proteins (orange (OFP), red (RFP), enhanced yellow (EYFP), and cyan (CFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.1 coding region contained (from 5 to 3) a LoxN site, Lox2272 site, LoxPsite, Kusabira-Orange OFP (kOFP; with polyA sequence), LoxN site,mCherry RFP (with membrane tethering palmitoylation sequence and polyAsequence), Lox2272 site, monomeric EYFP (mYFPA206K; with membrane tethering palmitoylation sequence and polyA sequence), LoxPsite, mCerulean CFP (with membrane tethering palmitoylation sequence and 5 FRTsite and polyA sequence). The construct was inserted into a BAC encoding the Slc1a3 gene via homologous recombination (at the ATG site). (J:282572)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:282572 Nakagawa N, et al., Memo1-Mediated Tiling of Radial Glial Cells Facilitates Cerebral Cortical Development. Neuron. 2019 Sep 4;103(5):836-852.e5
• All: 1 reference(s)