Hapln1^em2Mbp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hapln1^em2Mbp
• Name: hyaluronan and proteoglycan link protein 1; endonuclease-mediated mutation 2, Mouse Biology Program, UC Davis
• MGI ID: MGI:7606965
• Gene: Hapln1 Location: Chr13:89688654-89759951 bp, + strand Genetic Position: Chr13, 45.5 cM
• Alliance: Hapln1^em2Mbp page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: The mouse G388S model was created using optimized CRISPR Cas9 KI technology utilizing Ribonucleoprotein (RNP) in the presence of a synthetic single strand DNA repair template harboring the desired KI. Zygotes were electroporated and subsequent progeny were screened for the presence of the correctly targeted allele via homology directed repair (HDR). The strategy for editing the variant ablates all PAM types (2 of the 3 KI nucleotides [GG>TC] are part of the PAM), and protects the HDR-derived locus from re-cutting by Cas9 (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hapln1 Mutation: 27 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)