Calr^{tm1(CALR*)Argr}
Targeted Allele Detail

Summary

• Symbol: Calr^{tm1(CALR*)Argr}
• Name: calreticulin; targeted mutation 1, Anthony R Green
• MGI ID: MGI:7593751
• Synonyms: CALR^fl
• Gene: Calr Location: Chr8:85568717-85573560 bp, - strand Genetic Position: Chr8, 41.21 cM
• Alliance: Calr^{tm1(CALR*)Argr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262170
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutation: Insertion
• Calr^{tm1(CALR*)Argr} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CALR (811)

  Calr	(MGI:88252)

  cDNA with a 52 bp deletion found in patients with essential thrombocythemia and primary myelofibrosis

• Mutation details: A loxP site was inserted upstream of exon 8 (position 19478), and a pgk-neo-polyA cassette, loxP site, and mouse exon 8 fused to a human CALR cDNA with a 52 bp deletion followed by the mouse 3' UTR was inserted downstream of exon 9. (J:262170)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Calr Mutation: 15 strains or lines available

References

• Original: J:262170 Li J, et al., Mutant calreticulin knockin mice develop thrombocytosis and myelofibrosis without a stem cell self-renewal advantage. Blood. 2018 Feb 8;131(6):649-661
• All: 4 reference(s)