Tg(Cd2-TcrbSB14-31)EF4.1Kass
Transgene Detail

Summary

• Symbol: Tg(Cd2-TcrbSB14-31)EF4.1Kass
• Name: transgene insertion EF4.1, George Kassiotis
• MGI ID: MGI:7581840
• Transgene: Tg(Cd2-TcrbSB14-31)EF4.1Kass Location: unknown
• Alliance: Tg(Cd2-TcrbSB14-31)EF4.1Kass page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The SB14-31 T cell clone , specific to env122141 presented by MHC II Ab, was fused to the TCRaslphabeta-negative BW5147 thymoma cells to produce the 1A6 hybridoma cell line, which was then used as source for RNA. TCRalpha and TCRbeta cDNAs were cloned and inserted into the hCD2-VA expression cassette, which directs expression in T cells. Specifically for the TCRbeta chain, a cDNA-genomic DNA hybrid was used, in which intron 1 of the TCRVbeta-encoding gene (Tcrbv1s1) was preserved. For the present study, a TCR-transgenic line (EF4.1) expressing only the TCRbeta chain of the SB14-31 clone was selected, to maintain a polyclonal TCR repertoire. (J:260560)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:260560 Antunes I, et al., Retrovirus-specificity of regulatory T cells is neither present nor required in preventing retrovirus-induced bone marrow immune pathology. Immunity. 2008 Nov 14;29(5):782-94
• All: 6 reference(s)