Dnase1^em1Lnit
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnase1^em1Lnit
• Name: deoxyribonuclease I; endonuclease-mediated mutation 1, Lars Nitschke
• MGI ID: MGI:7581011
• Gene: Dnase1 Location: Chr16:3855007-3857888 bp, + strand Genetic Position: Chr16, 2.37 cM, cytoband A1-A3
• Alliance: Dnase1^em1Lnit page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:345011
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology using sgRNA 5-TGACATCGCTGTTATCCAAG-3 generated a 65 bp deletion from intron 2 into exon 3. This deletion does not allow splicing into exon 3, and potential alternative splicing into exons 4, 5 or 6 containing the active sites for Dnase1 enzymatic activity lead to frameshift mutations and premature stop codons. (J:345011)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnase1 Mutation: 28 strains or lines available

References

• Original: J:345011 Korn MA, et al., Epistatic effects of Siglec-G and DNase1 or DNase1l3 deficiencies in the development of systemic lupus erythematosus. Front Immunol. 2023;14:1095830
• All: 1 reference(s)