Pax6^{em8(PAX6*R240X)Ems}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pax6^{em8(PAX6*R240X)Ems}
• Name: paired box 6; endonuclease-mediated mutation 8, Elizabeth M Simpson
• MGI ID: MGI:7579265
• Synonyms: Pax6^{em8(FLAG/PAX6*R240X)Ems}, Pax6^FHe9-
• Gene: Pax6 Location: Chr2:105499245-105527709 bp, + strand Genetic Position: Chr2, 55.31 cM
• Alliance: Pax6^{em8(PAX6*R240X)Ems} page

Mutation origin

• Strain of Origin: B6.Cg-Pax6^Sey/EmsMmmh

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Pax6^{em8(PAX6*R240X)Ems} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PAX6 (5080)

  Pax6	(MGI:97490)

• Mutation details: CRISPR-targeting of zygotes carrying Pax6^em3Ems allele harbors a triple FLAG tag at the ATG translation initiation sequence in exon 4 was used to replace mouse exon 9 with human exon 9 sequence that contains the human mutation (c.718C>T (p.Arg240Ter)). (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pax6 Mutation: 92 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)