Mlkl^em1Pme
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mlkl^em1Pme
• Name: mixed lineage kinase domain-like; endonuclease-mediated mutation 1, Pascal Meier
• MGI ID: MGI:7576921
• Synonyms: Mlkl^K219R
• Gene: Mlkl Location: Chr8:112038429-112064809 bp, - strand Genetic Position: Chr8, 57.98 cM, cytoband D3
• Alliance: Mlkl^em1Pme page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Lysine codon 219 (AAA) in exon 4 was changed to arginine (CGG) (p.K219R) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation blocks ubiquitylation of the residue in the encoded peptide. (J:343897)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mlkl Mutation: 34 strains or lines available

References

• Original: J:343897 Garcia LR, et al., Ubiquitylation of MLKL at lysine 219 positively regulates necroptosis-induced tissue injury and pathogen clearance. Nat Commun. 2021 Jun 7;12(1):3364
• All: 1 reference(s)