Rr467^em1Nses
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr467^em1Nses
• Name: regulatory region Rr467; endonuclease-mediated mutation 1, Nenad Sestan
• MGI ID: MGI:7576709
• Synonyms: hCbln2 E2 KI
• Gene: Rr467 Location: Chr18:86742014-86742297 bp Genetic Position: Chr18, Syntenic
• Alliance: Rr467^em1Nses page

Mutation origin

• Strain of Origin: (C57BL/6J x SJL/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Modified regulatory region)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 technology replaced one of the cis-regulatory elements marked as DNase I-hypersensitive sites around the mouse Cbln2 locus designated as the Cbln2 enhancer E2 (1,005 bp) with the corresponding human CBLN2 enhancer E2 (chr18: 72,530,47372,531,324, GRCh38/hg38). Quantitative RT-PCR showed increased RNA expression of mouse Cbln2 in neonatal neocortex in homozygous mice. (J:331714)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr467 Mutation: 0 strains or lines available

References

• Original: J:331714 Shibata M, et al., Hominini-specific regulation of CBLN2 increases prefrontal spinogenesis. Nature. 2021 Oct;598(7881):489-494
• All: 1 reference(s)