Enam^em2Xwang
Endonuclease-mediated Allele Detail

Summary

• Symbol: Enam^em2Xwang
• Name: enamelin; endonuclease-mediated mutation 2, Xiaofang Wang
• MGI ID: MGI:7573107
• Synonyms: ENAM S55D
• Gene: Enam Location: Chr5:88635834-88653908 bp, + strand Genetic Position: Chr5, 43.66 cM, cytoband E2
• Alliance: Enam^em2Xwang page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Serine codon 55 (AGT) in exon 5 was changed to aspartic acid (GAT) (p.S55D) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation changes the phosphorylatable residue in the encoded peptide to a phosphomimetic. (J:342731)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Enam Mutation: 71 strains or lines available

References

• Original: J:342731 Dong C, et al., The phosphorylation of serine(55) in enamelin is essential for murine amelogenesis. Matrix Biol. 2022 Jul 9
• All: 1 reference(s)