Setd4^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Setd4^{tm1d(KOMP)Wtsi}
• Name: SET domain containing 4; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7571179
• Gene: Setd4 Location: Chr16:93380345-93400951 bp, - strand Genetic Position: Chr16, 54.52 cM
• Alliance: Setd4^{tm1d(KOMP)Wtsi} page
• IMPC: Setd4 gene page

Mutation origin

• Mutant Cell Line: EPD0225_5_D10
• Germline Transmission: Earliest citation of germline transmission: J:343786
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 93389900 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 93390710. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette. Cre-mediated recombination removed the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:343786)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Setd4 Mutation: 32 strains or lines available

References

• Original: J:343786 Lana-Elola E, et al., Congenital heart defects in Down syndrome are caused by increased dosage of DYRK1A. bioRxiv. 2023
• All: 2 reference(s)