Crisp2^tm1Osb
Targeted Allele Detail

Summary

• Symbol: Crisp2^tm1Osb
• Name: cysteine-rich secretory protein 2; targeted mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:7570125
• Synonyms: Crisp2^{tm1a(KOMP)Osb}
• Gene: Crisp2 Location: Chr17:41075625-41105037 bp, - strand Genetic Position: Chr17, 19.54 cM
• Alliance: Crisp2^tm1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280685
• Parent Cell Line: EGR-G101 (ES Cell)
• Strain of Origin: C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Using targeting vector (PG00061_Y_6_A09), the L1L2_Bact_P cassette was inserted at position 41095297 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 41096404. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:280685)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crisp2 Mutation: 13 strains or lines available

References

• Original: J:280685 Brukman NG, et al., Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders. Mol Hum Reprod. 2016 Apr;22(4):240-51
• All: 1 reference(s)