P2ry1^em1Robn
Endonuclease-mediated Allele Detail

Summary

• Symbol: P2ry1^em1Robn
• Name: purinergic receptor P2Y, G-protein coupled 1; endonuclease-mediated mutation 1, Robert A Nicholas
• MGI ID: MGI:7569091
• Synonyms: P2Y1^340-0P
• Gene: P2ry1 Location: Chr3:60910216-60916403 bp, + strand Genetic Position: Chr3, 29.24 cM, cytoband E1
• Alliance: P2ry1^em1Robn page

Mutation origin

• Strain of Origin: C57BL/6NHsd

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Sequence coding for an HA tag was inserted immediately after the start codon and all six serine (TCC, AGT, TCC, AGT, TCT, AGT) and two threonine (ACT, ACG) codons within the sequence coding for the C-terminal tail were mutated to alanine codons (GCC, GCT, GCC, GCT, GCT, GCT) (GCT, GCG) (p.S343A, p.S346A, p.S352A, p.S354A, p.T358A, p.S363A, p.T371_S372delinsAA) using sgRNAs (targeting GGAAAGAAGAGGATGACCG and TGGAGACACGAGTTTGTGA) and ssODN templates with CRISPR/Cas9 technology. The mutations (SRRSEANLOSKSEDMTLNILSEFKONGDTSL to ARRAEANLQAKAEDMALNILAEFKQNGDAAL) block phosphorylation of the encoded residues and prevent desensitization of the encoded peptide in platelets. (J:342773)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any P2ry1 Mutation: 31 strains or lines available

References

• Original: J:342773 Paul DS, et al., Loss of P2Y(1) receptor desensitization does not impact hemostasis or thrombosis despite increased platelet reactivity in vitro. J Thromb Haemost. 2023 Jul;21(7):1891-1902
• All: 1 reference(s)