Mstn^{em2(Gdf11)Rlee}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mstn^{em2(Gdf11)Rlee}
• Name: myostatin; endonuclease-mediated mutation 2, Richard T Lee
• MGI ID: MGI:7568819
• Synonyms: Gdf8^11MD, Gdf8^Gdf11MD
• Gene: Mstn Location: Chr1:53100799-53107238 bp, + strand Genetic Position: Chr1, 26.99 cM
• Alliance: Mstn^{em2(Gdf11)Rlee} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The sequence coding for the mature domain (aspartic acid codon 268 through serine codon 376) in exon 3 was replaced with the equivalent sequence from paralog Gdf11 (asparagine codon 297 through serine codon 405) using sgRNAs and a dsODN template with CRISPR/Cas9 technology. (J:342779)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mstn Mutation: 38 strains or lines available

References

• Original: J:342779 Lian J, et al., Functional substitutions of amino acids that differ between GDF11 and GDF8 impact skeletal development and skeletal muscle. Life Sci Alliance. 2023 Mar;6(3)
• All: 1 reference(s)