Mstn^em1Rlee
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mstn^em1Rlee
• Name: myostatin; endonuclease-mediated mutation 1, Richard T Lee
• MGI ID: MGI:7568816
• Synonyms: Gdf8^11aa, Gdf8^Gdf11aa
• Gene: Mstn Location: Chr1:53100799-53107238 bp, + strand Genetic Position: Chr1, 26.99 cM
• Alliance: Mstn^em1Rlee page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Glycine codon 356 (GGC) in exon 3 was changed to aspartic acid (GAC) (p.G356D) and glutamic acid codon 358 (GAA) to glutamine (CAA) (p.E358Q) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutations change the endogenous amino acids in the encoded peptide to their equivalents from paralog Gdf11. (J:342779)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mstn Mutation: 38 strains or lines available

References

• Original: J:342779 Lian J, et al., Functional substitutions of amino acids that differ between GDF11 and GDF8 impact skeletal development and skeletal muscle. Life Sci Alliance. 2023 Mar;6(3)
• All: 1 reference(s)