Mecp2^tm4.1Meg
Targeted Allele Detail

Summary

• Symbol: Mecp2^tm4.1Meg
• Name: methyl CpG binding protein 2; targeted mutation 4.1, Michael E Greenberg
• MGI ID: MGI:7567375
• Synonyms: MeCP2 QKI
• Gene: Mecp2 Location: ChrX:73070198-73129296 bp, - strand Genetic Position: ChrX, 37.63 cM
• Alliance: Mecp2^tm4.1Meg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342941
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Serine codons 86 in exon 3 (TCC) and 274 (AGT) and 421 (TCA) in exon 4, and threonine codon 308 (ACC) in exon 4 were changed to alanine (GCT, GCT, GCA, GCC) (p.S86A, p.S274A, p.S421A, p.T308A) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 3. The neo cassette was removed through subsequent Cre-mediated recombination. The mutations prevent activity-induced phosphorylation of the affected residues in the encoded peptide. (J:342941)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mecp2 Mutation: 46 strains or lines available

References

• Original: J:342941 Tzeng CP, et al., Activity-induced MeCP2 phosphorylation regulates retinogeniculate synapse refinement. Proc Natl Acad Sci U S A. 2023 Oct 31;120(44):e2310344120
• All: 2 reference(s)