Apoe^{em1(APOE_i4*)Yhg}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Apoe^{em1(APOE_i4*)Yhg}
• Name: apolipoprotein E; endonuclease-mediated mutation 1, Yadong Huang
• MGI ID: MGI:7565501
• Synonyms: APOE4-R136S-KI
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^{em1(APOE_i4*)Yhg} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Single point mutation
• Mutation details: CRISPR/Cas9 technology introduced a C-to-A change resulting in an arginine to serine substitution at amino acid 136 (p.R136S) in the inserted human APOE isoform 4 of the Apoe^{tm3(APOE_i4)Yhg} allele. (J:343279)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 164 strains or lines available

Notes

Zygotes used for targeting were generated by mating superovulated C57BL/6 females to Apoe^{tm3(APOE_i4)Yhg} males.

References

• Original: J:343279 Nelson MR, et al., The APOE-R136S mutation protects against APOE4-driven Tau pathology, neurodegeneration and neuroinflammation. Nat Neurosci. 2023 Dec;26(12):2104-2121
• All: 1 reference(s)