Kmt2d^em1Kaig
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kmt2d^em1Kaig
• Name: lysine (K)-specific methyltransferase 2D; endonuclease-mediated mutation 1, Kai Ge
• MGI ID: MGI:7565499
• Synonyms: Mll4^KI, MLL4 Y5477A
• Gene: Kmt2d Location: Chr15:98729550-98769085 bp, - strand
• Alliance: Kmt2d^em1Kaig page

Mutation origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1/J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Tyrosine codon 5477 (TAC) in exon 52 was changed to alanine (GCT) (p.Y5477A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation creates a enzyme-dead version of the encoded peptide. (J:342639)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmt2d Mutation: 169 strains or lines available

References

• Original: J:342639 Xie G, et al., MLL3/MLL4 methyltransferase activities control early embryonic development and embryonic stem cell differentiation in a lineage-selective manner. Nat Genet. 2023 Apr;55(4):693-705
• All: 1 reference(s)