Srcap^em1Mago
Endonuclease-mediated Allele Detail

Summary

• Symbol: Srcap^em1Mago
• Name: Snf2-related CREBBP activator protein; endonuclease-mediated mutation 1, Margaret A Goodell
• MGI ID: MGI:7564597
• Synonyms: Srcap^mut, Srcap^Q1963X
• Gene: Srcap Location: Chr7:127111155-127160391 bp, + strand Genetic Position: Chr7, 69.56 cM
• Alliance: Srcap^em1Mago page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: Glutamine codon 1963 (CAG) in exon 26 was changed to a stop codon (TAG) (p.Q1963*) and a sequence coding for a 3x FLAG tag was inserted immediately upstream of codon 1963 using an sgRNA and an ssODN template with CRISPR/Cas9 technology. (J:342674)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Srcap Mutation: 135 strains or lines available

References

• Original: J:342674 Chen CW, et al., SRCAP mutations drive clonal hematopoiesis through epigenetic and DNA repair dysregulation. Cell Stem Cell. 2023 Nov 2;30(11):1503-1519.e8
• All: 1 reference(s)