Gt(ROSA)26Sor^{tm1(CAG-mEGFP,-YFP*)Cheny}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-mEGFP,-YFP*)Cheny}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Yao Chen
• MGI ID: MGI:7563916
• Synonyms: FL-AK, FLIM-AKAR^flox
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-mEGFP,-YFP*)Cheny} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:349008
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac x C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct contains a CAG-reversed [loxP-pA-PGK-Neomycin-STOP-loxP]-FLIM-AKAR-WPRE cassette was introduced to intron 1. The Fluorescence Lifetime Imaging Microscopy compatible A Kinase Activity Reporter (FLIM-AKAR) optical reporter (based on the synthetic reporter found in Addgene plasmid# 63058) consists of a donor fluorophore of monomeric enhanced green fluorescent protein (mEGFP) and an acceptor fluorophore of dark yellow fluorescent protein (sREACH). (J:349008)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:349008 Tilden EI, et al., A Cre-dependent reporter mouse for quantitative real-time imaging of protein kinase A activity dynamics. Sci Rep. 2024 Feb 6;14(1):3054
• All: 1 reference(s)