Stat3^em1Mand
Endonuclease-mediated Allele Detail

Summary

• Symbol: Stat3^em1Mand
• Name: signal transducer and activator of transcription 3; endonuclease-mediated mutation 1, Mark S Anderson
• MGI ID: MGI:7562553
• Synonyms: STAT3^K392R
• Gene: Stat3 Location: Chr11:100777632-100830447 bp, - strand Genetic Position: Chr11, 63.82 cM
• Alliance: Stat3^em1Mand page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Constitutively active, Humanized sequence)
• Mutation: Single point mutation
• Mutation details: Lysine codon 392 (AAA) in exon 13 was changed to arginine (AGA) (p.K392R) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation, in the DNA-binding domain of the encoded peptide, represents the same human gain-of-function mutation (NM_011486.3:c.1175A>G) that affects diabetes severity. (J:342716)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stat3 Mutation: 72 strains or lines available

References

• Original: J:342716 Warshauer JT, et al., A human mutation in STAT3 promotes type 1 diabetes through a defect in CD8+ T cell tolerance. J Exp Med. 2021 Aug 2;218(8)
• All: 1 reference(s)