Idh1^tm1.1Avd
Targeted Allele Detail

Summary

• Symbol: Idh1^tm1.1Avd
• Name: isocitrate dehydrogenase 1 (NADP+), soluble; targeted mutation 1.1, Andreas von Deimling
• MGI ID: MGI:7562323
• Synonyms: Idh1tm1(R132H)Avd
• Gene: Idh1 Location: Chr1:65197775-65225638 bp, - strand Genetic Position: Chr1, 32.91 cM
• Alliance: Idh1^tm1.1Avd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342699
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site was inserted into intron 2, and a lox511 site, and, in reverse orientation, a copy of exon 3 (where arginine codon 132 (CGA) was changed to histidine (p.R132H)), a second loxP site and a second lox511 site, were inserted into intron 3. An FRT site flanked selection cassette that was inserted between the second loxP and lox511 sites was removed through subsequent Flp-mediated recombination. This allele expresses the wildtype peptide and only after conditional Cre-mediated flipping of the mutated exon copy and removal of the wildtype exon will it express the modified peptide. The mutation represents the most common variant found in human gliomas. (J:342699)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Idh1 Mutation: 40 strains or lines available

References

• Original: J:342699 Bunse L, et al., Suppression of antitumor T cell immunity by the oncometabolite (R)-2-hydroxyglutarate. Nat Med. 2018 Aug;24(8):1192-1203
• All: 1 reference(s)