Ackr3^tm2.1Stum
Targeted Allele Detail

Summary

• Symbol: Ackr3^tm2.1Stum
• Name: atypical chemokine receptor 3; targeted mutation 2.1, Ralf Stumm
• MGI ID: MGI:7562045
• Synonyms: Ackr3^STA, HA-Ackr3^STA, HA-ACKR3-ST/A
• Gene: Ackr3 Location: Chr1:90131702-90143446 bp, + strand Genetic Position: Chr1, 45.28 cM
• Alliance: Ackr3^tm2.1Stum page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275611
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Epitope tag)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Sequence coding for an HA tag was inserted immediately downstream of the ATG start codon in exon 2, a loxP site flanked neomycin resistance gene cassette was inserted into intron 1 and the following mutations were engineered in exon 2: serine codons 535 (TCG), 347 (TCC), 350 (TCA) and 355 (TCT) and threonine codons 338 (ACA), 341 (ACC), 352 (ACA) and 361 (ACC) were changed to alanine (p.S335A, p.S347A, p.S350A, p.S355A, p.T338A, p.T341A, p.T352A and p.T361A). The neo cassette was removed through subsequent Cre-mediated recombination. The mutations block phosphorylation of the affected residues in the encoded peptide. (J:275611)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ackr3 Mutation: 34 strains or lines available

References

• Original: J:275611 Saaber F, et al., ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not beta-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9
• All: 2 reference(s)