Pth1r^{tm1.1(PTH1R)Tjg}
Targeted Allele Detail

Summary

• Symbol: Pth1r^{tm1.1(PTH1R)Tjg}
• Name: parathyroid hormone 1 receptor; targeted mutation 1.1, Thomas J Gardella
• MGI ID: MGI:7549298
• Synonyms: hPTH1R-KI
• Gene: Pth1r Location: Chr9:110551153-110576213 bp, - strand Genetic Position: Chr9, 60.56 cM, cytoband F
• Alliance: Pth1r^{tm1.1(PTH1R)Tjg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:340805
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Pth1r^{tm1.1(PTH1R)Tjg} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PTH1R (5745)

  Pth1r

  (MGI:97801)

• Mutation details: Exon 4 and a 5 end portion of intron 4 were replaced by a cassette containing the cDNA for human PTH1R residues Val-26 to Met-593 with an HA tag replacing the segment Tyr88-Glu96, followed by a TGA stop codon and a transcription termination/polyadenylation sequence from the rabbit beta globin gene and then a self-deleting neomycin resistance gene, which was self-excised. Splicing of the expressed transcript results in mouse exon 3 being joined at the codon corresponding to Leu25 in the mouse Pth1r to the codon for Val26 in the human cDNA sequence. Mouse exon 3 encodes residues Met1-Leu25, which includes the signal sequence. (J:340805)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pth1r Mutation: 27 strains or lines available

References

• Original: J:340805 Daley EJ, et al., Actions of Parathyroid Hormone Ligand Analogues in Humanized PTH1R Knockin Mice. Endocrinology. 2022 Jul 1;163(7)
• All: 1 reference(s)