Ripk1^em1Yuan
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ripk1^em1Yuan
• Name: receptor (TNFRSF)-interacting serine-threonine kinase 1; endonuclease-mediated mutation 1, Junying Yuan
• MGI ID: MGI:7548564
• Synonyms: Ripk1^K612R
• Gene: Ripk1 Location: Chr13:34186346-34221130 bp, + strand Genetic Position: Chr13, 14.01 cM
• Alliance: Ripk1^em1Yuan page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Lysine codon 612 (AAA) in exon 11 was changed to arginine (CGC) (p.K612R) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation interferes with ubiquitination and inhibits activation of the encoded peptide. (J:301417)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ripk1 Mutation: 31 strains or lines available

References

• Original: J:301417 Li X, et al., Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners. Nat Commun. 2020 Dec 11;11(1):6364
• All: 1 reference(s)