Btk^em1Mnz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Btk^em1Mnz
• Name: Bruton agammaglobulinemia tyrosine kinase; endonuclease-mediated mutation 1, Michel C Nussenzweig
• MGI ID: MGI:7548531
• Synonyms: BTK^C481S
• Gene: Btk Location: ChrX:133443085-133484319 bp, - strand Genetic Position: ChrX, 56.18 cM
• Alliance: Btk^em1Mnz page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Single point mutation
• Mutation details: Cysteine codon 481 (TGC) in exon 15 was changed to serine (TCC) (p.C481S) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation renders the encoded peptide insensitive to B-cell proliferation and survival inhibitor ibrutinib. (J:340979)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Btk Mutation: 22 strains or lines available

References

• Original: J:340979 Chen ST, et al., B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection. Immunity. 2023 Mar 14;56(3):547-561.e7
• All: 1 reference(s)