Adar^em2Crwy
Endonuclease-mediated Allele Detail

Summary

• Symbol: Adar^em2Crwy
• Name: adenosine deaminase, RNA-specific; endonuclease-mediated mutation 2, Carl R Walkley
• MGI ID: MGI:7547459
• Synonyms: Adar1^-, Adar1^L196Cfs
• Gene: Adar Location: Chr3:89622329-89660753 bp, + strand Genetic Position: Chr3, 39.19 cM, cytoband F2
• Alliance: Adar^em2Crwy page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Proline codon 195 (CCT) in exon 2 was targeted for change to alanine (GCT) (p.P195A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. This allele represents an unintended deletion event where a single T nucleotide in exon 2 was deleted (c.587delT), resulting in a frameshift and premature stop codon (p.L196Cfs*7); the P195 codon did not change. This mutation affects the translation of the p150 isoform but not the p110 isoform. (J:342175)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adar Mutation: 83 strains or lines available

References

• Original: J:342175 Liang Z, et al., Generation of a new Adar1p150 (-/-) mouse demonstrates isoform-specific roles in embryonic development and adult homeostasis. RNA. 2023 Sep;29(9):1325-1338
• All: 2 reference(s)