Gt(ROSA)26Sor^{tm4(Akt1*E17K)Jrld}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(Akt1*E17K)Jrld}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Jurgen Ruland
• MGI ID: MGI:7547005
• Synonyms: LSL-Akt^E17K
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(Akt1*E17K)Jrld} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:298712
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm4(Akt1*E17K)Jrld} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Akt1	Contains E17K mutation rendering consitutive activity

• Mutation details: A loxP-flanked stop cassette (LSL) and Akt1 cDNA containing a glutamic acid to lysine substitution at amino acid 17 (p.E17K) with an N-terminal Flag tag were inserted into the locus. The pE17K mutation renders constitutive activation of AKT1. Cre mediated recombination is required to induce expression of the constitutively active AKT1. (J:298712)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 956 strains or lines available

References

• Original: J:298712 Varga J, et al., AKT-dependent NOTCH3 activation drives tumor progression in a model of mesenchymal colorectal cancer. J Exp Med. 2020 Oct 5;217(10)
• All: 1 reference(s)