Tmem106b^{tm2d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem106b^{tm2d(KOMP)Wtsi}
• Name: transmembrane protein 106B; targeted mutation 2d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7546876
• Gene: Tmem106b Location: Chr6:13069758-13089268 bp, + strand Genetic Position: Chr6, 5.38 cM, cytoband A2
• Alliance: Tmem106b^{tm2d(KOMP)Wtsi} page
• IMPC: Tmem106b gene page

Mutation origin

• Mutant Cell Line: EPD0047_1_E02
• Germline Transmission: Earliest citation of germline transmission: J:342155
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 13074587 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 13075361. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:342155)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem106b Mutation: 29 strains or lines available

References

• Original: J:342155 Nguyen Q, et al., The TMEM106B T186S coding variant increases neurite arborization and synaptic density in primary hippocampal neurons. Front Neurosci. 2023;17:1275959
• All: 2 reference(s)