Gt(ROSA)26Sor^{tm3.1(CAG-Venus,-Apex2)Jrs}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3.1(CAG-Venus,-Apex2)Jrs}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3.1, Joshua R Sanes
• MGI ID: MGI:7546839
• Synonyms: RC-sV-A
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3.1(CAG-Venus,-Apex2)Jrs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341917
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm3.1(CAG-Venus,-Apex2)Jrs} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Apex2 (MGI:1924872)	nulcear localization signal

• Mutation details: A targeting vector is designed with (from 5' to 3') a ubiquitously expressed CAG promoter, a loxP-flanked stop cassette, a Venus reporter sequence, 3F2A (three tandem repeats of foot-and-mouth disease 2A peptide sequence) that mediates ribosomal skipping, the Apex2 gene sequence with a nuclear localization signal, a WPRE sequence, and an FRT-flanked neomycin resistance (neo) cassette. Flp-mediated recombination removed the neo cassette. (J:341917)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:341917 Yamagata M, et al., Expression and Roles of the Immunoglobulin Superfamily Recognition Molecule Sidekick1 in Mouse Retina. Front Mol Neurosci. 2018;11:485
• All: 1 reference(s)