Marchf5^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Marchf5^{tm1c(EUCOMM)Wtsi}
• Name: membrane associated ring-CH-type finger 5; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7546820
• Synonyms: March5^fl
• Gene: Marchf5 Location: Chr19:37184942-37199550 bp, + strand Genetic Position: Chr19, 32.2 cM
• Alliance: Marchf5^{tm1c(EUCOMM)Wtsi} page
• IMPC: Marchf5 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342139
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 37194086 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 37194938. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:342139)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Marchf5 Mutation: 16 strains or lines available

References

• Original: J:342139 Park YJ, et al., MARCH5-dependent NLRP3 ubiquitination is required for mitochondrial NLRP3-NEK7 complex formation and NLRP3 inflammasome activation. EMBO J. 2023 Oct 4;42(19):e113481
• All: 1 reference(s)