Gys1^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gys1^{tm1d(EUCOMM)Wtsi}
• Name: glycogen synthase 1, muscle; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:7545178
• Gene: Gys1 Location: Chr7:45084268-45106043 bp, + strand Genetic Position: Chr7, 29.31 cM
• Alliance: Gys1^{tm1d(EUCOMM)Wtsi} page
• IMPC: Gys1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:334420
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_gt1 cassette was inserted at position 45091398 of Chromosome 7 upstream of the critical exons 6-8 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons 6-8 at position 45093292. The critical exons 6-8 are thus flanked by loxP sites. Flp mediated recombination created the "conditional ready" (floxed). Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:334420)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gys1 Mutation: 56 strains or lines available

References

• Original: J:334420 Mitra S, et al., Laforin targets malin to glycogen in Lafora progressive myoclonus epilepsy. Dis Model Mech. 2023 Jan 1;16(1)
• All: 1 reference(s)