Gt(ROSA)26Sor^{em1.1(CAG-Casp3)Ddg}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1.1(CAG-Casp3)Ddg}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, David D Ginty
• MGI ID: MGI:7540317
• Synonyms: R26^{FSF-LSL-Caspase3}, ROSA26^{FSF-LSL-Caspase3}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1.1(CAG-Casp3)Ddg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341426
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Using standard CRISPR-based homologous recombination techniques in embryonic stem (ES) cells, CAG promoter-Frt-Stop-Frt followed by lox-Stop-lox and Casp3 expression cassette were inserted into the Gt(ROSA)26Sor locus. Chimeras were generated by blastocyst injection and subsequent germline transmission was confirmed by tail PCR. The neo-selection cassette was excised using a phiC31 strain. (J:101977, J:341426)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:341426 Wolfson RL, et al., DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon. Cell. 2023 Aug 3;186(16):3368-3385.e18
• All: 2 reference(s)