Tg(NPHS2-CASP8*)1Pesch
Transgene Detail

Summary

• Symbol: Tg(NPHS2-CASP8*)1Pesch
• Name: transgene insertion 1, Philipp Scherer
• MGI ID: MGI:7539687
• Synonyms: POD-ATTAC
• Transgene: Tg(NPHS2-CASP8*)1Pesch Location: unknown
• Alliance: Tg(NPHS2-CASP8*)1Pesch page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(NPHS2-CASP8*)1Pesch expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CASP8 (841)

  Casp8

  (MGI:1261423)

  Expresses human caspase 8 fused to serial FKBPv (Phe36Val mutant FKBP) dimerization domains

• Tg(NPHS2-CASP8*)1Pesch expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  NPHS2 (7827)

  Nphs2

  (MGI:2157018)

• Mutation details: The 2.5-kb human podocin promoter (Nphs2) drives expression of human caspase 8 fused to serial FKBPv (Phe36Val mutant FKBP) dimerization domains. A myristoylation site was included to provide membrane attachment for the caspase 8/FKBP fusion protein. Expression of the fusion protein under the control of the Nphs2 podocyte specific promoter, together with treatment to induce dimerization of the FKBP domains, results in widespread apoptosis of podocytes. Four transgenic lines were generated, but one line showed high expression and was studied further. (J:321645)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:321645 Rutkowski JM, et al., Adiponectin promotes functional recovery after podocyte ablation. J Am Soc Nephrol. 2013 Feb;24(2):268-82
• All: 10 reference(s)