Igh^tm5.1Mbu
Targeted Allele Detail

Summary

• Symbol: Igh^tm5.1Mbu
• Name: immunoglobulin heavy chain complex; targeted mutation 5.1, Meinrad Busslinger
• MGI ID: MGI:7539580
• Synonyms: Igh^P4-Bsd
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341427
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, RMCE-ready)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CreERT2 activity was induced by 4-hydroxytamoxifen to facilitate Cre-mediated insertion of the PAIR4 sequences into the lox71 site at the deletion point (117,126,667/116,237,220; mm9, Chr.12) of the Igh^tm4.2Mbu allele. The rox-flanked Actb-Bsd expression cassette was deleted after germline transmission. (J:341427)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 47 strains or lines available

Notes

Blastocyts from Igh^tm4.2Mbu/+ Rosa26CreERT2/+ mice on Gt(ROSA)26Sor^{tm9(cre/ESR1)Arte} on a hybrid C57BL/6 129/Sv background were used to generate ES cell lines.

References

• Original: J:341427 Hill L, et al., Enhancers of the PAIR4 regulatory module promote distal V(H) gene recombination at the Igh locus. EMBO J. 2023 Aug 1;42(15):e112741
• All: 1 reference(s)