Igh^tm4.2Mbu
Targeted Allele Detail

Summary

• Symbol: Igh^tm4.2Mbu
• Name: immunoglobulin heavy chain complex; targeted mutation 4.2, Meinrad Busslinger
• MGI ID: MGI:7539574
• Synonyms: Igh^delta890
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294166
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The allele was generated by Cre-mediated deletion of 890kb genomic sequence of the lox71/loxP-flanked Igh region in Igh^tm4Mbu. The frt flanked Pgk1 promoter region was removed by Flpe recombinase in Igh^tm4.1Mbu. The allele contains only a promoterless blasticidin resistance gene at the deletion point. (J:294166)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 47 strains or lines available

References

• Original: J:294166 Hill L, et al., Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion. Nature. 2020 Aug;584(7819):142-147
• All: 2 reference(s)