Stxbp1^tm1.1Wagr
Targeted Allele Detail

Summary

• Symbol: Stxbp1^tm1.1Wagr
• Name: syntaxin binding protein 1; targeted mutation 1.1, Wade G Regehr
• MGI ID: MGI:7539573
• Synonyms: Munc18-1SA
• Gene: Stxbp1 Location: Chr2:32677619-32737249 bp, - strand Genetic Position: Chr2, 22.09 cM
• Alliance: Stxbp1^tm1.1Wagr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:339829
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv x C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 11 and an FRT site flanked neomycin resistance gene cassette, a lox2272 site, a mutated copy of exon 11 in reverse orientation, a second loxP site and a second lox2272 site (both in opposite orientation compared to respective first sites) were inserted into intron 11. The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses the wildtype transcript/peptide and only after Cre-mediated flipping of the mutated exon 11 and concurrent deletion of the WT exon 11 will it express the mutated transcript/peptide. The mutation involves changing serine codons 306 (TCT), 312 (TCT) and 313 (AGC) with alanine codons (GCT, GCT, GCC) to block phosphorylation of the three residues in the encoded peptide. (J:339829)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stxbp1 Mutation: 46 strains or lines available

References

• Original: J:339829 Wang CC, et al., Presynaptic Short-Term Plasticity Persists in the Absence of PKC Phosphorylation of Munc18-1. J Neurosci. 2021 Sep 1;41(35):7329-7339
• All: 1 reference(s)