Mlc1^tm1.1Jhmc
Targeted Allele Detail

Summary

• Symbol: Mlc1^tm1.1Jhmc
• Name: megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human); targeted mutation 1.1, Joseph H McCarty
• MGI ID: MGI:7537023
• Synonyms: Mlc1-eGFP
• Gene: Mlc1 Location: Chr15:88840087-88863192 bp, - strand Genetic Position: Chr15, 44.46 cM
• Alliance: Mlc1^tm1.1Jhmc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252682
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The T2A-EGFP cDNA was inserted in the open reading frame of the gene after the CAG codon in exon 12 such that the ribosome will translate through the T2A-eGFP sequence, resulting in two expressed proteins (Mlc1 and eGFP). (J:252682)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mlc1 Mutation: 28 strains or lines available

References

• Original: J:252682 Toutounchian JJ, et al., Selective expression of eGFP in mouse perivascular astrocytes by modification of the Mlc1 gene using T2A-based ribosome skipping. Genesis. 2017 Oct;55(10)
• All: 3 reference(s)