Tg(H2-T18*a-3)2Yuo
Transgene Detail

Summary

• Symbol: Tg(H2-T18*a-3)2Yuo
• Name: transgene insertion 2, Yuichi Obata
• MGI ID: MGI:7531446
• Synonyms: Tg.Tlaa-3-2
• Transgene: Tg(H2-T18*a-3)2Yuo Location: unknown
• Alliance: Tg(H2-T18*a-3)2Yuo page

Transgene origin

• Strain of Origin: C3H/HeNCrj

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene, derived from a clone isolated from an A-strain thymocyte library, contains a 9.6-kb mouse genomic DNA fragment that encompasses the H2-T18^a haplotype gene encoding the TLA^a-3 antigen. Transgene-derived transcripts and antigen are expressed at high levels in thymus and at lower levels in spleen and lymph nodes, in contrast with the thymus-restricted expression seen in endogenously TL-expressing mouse strains; no expression was detected in bone marrow. The primary transcript, of 2.4 kb, is seen as the minor mRNA species in thymi of strain A (H2-T18^a) mice and of C57BL/6 mice congenic for the H2-T18^a haplotype. (J:118591)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two lines of transgenic mice were generated by microinjection of this DNA fragment into fertilized oocytes of strain C3H/HeNCrj, chosen because C3H/He mice do not express H2-T18/TLA antigen from their endogenous H2-T18 gene(s). The line designated Tg.Tla^a-3-1, estimated to carry 7 tandemly-arrayed copies of the transgene, exhibits much higher thymic expression than does line Tg.Tla^a-3-2, with 12 copies. The latter line fails to develop the thymic abnormalities or cancer phenotype of the former.

References

• Original: J:118591 Hamasima N, et al., Expression of TL, H-2, and chimeric H-2/TL genes in transgenic mice: abnormal thymic differentiation and T-cell lymphomas in a TL transgenic strain. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7995-9
• All: 1 reference(s)