Gt(ROSA)26Sor^{tm2(CAG-GFPT1*G451E)Xen}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-GFPT1*G451E)Xen}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Taconic Biosciences
• MGI ID: MGI:7530916
• Synonyms: huGFAT1 G451E OE
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-GFPT1*G451E)Xen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:340718
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Gt(ROSA)26Sor^{tm2(CAG-GFPT1*G451E)Xen} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GFPT1 (2673)

  Gfpt1

  (MGI:95698)

  Contains a p.G451E mutation

• Mutation details: The targeting construct consisting of an F3 site, a neomycin resistance cassette, CAG promoter, loxP-flanked transcriptional termination cassette, FLAG-HA tagged human GFAT1 open reading frame containing a glycine to glutamate substitution at residue 451, human growth hormone polyadenylation signal and additional polyadenylation signal and FRT site were inserted into the locus. Upon cre-mediated recombination, the STOP cassette can be removed to get expression of the mutant human GFAT1 under the control of the CAG promoter. (J:340718)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1083 strains or lines available

References

• Original: J:340718 Allmeroth K, et al., Hexosamine pathway activation improves memory but does not extend lifespan in mice. Aging Cell. 2022 Oct;21(10):e13711
• All: 1 reference(s)