Sh3pxd2a^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sh3pxd2a^{tm1c(EUCOMM)Wtsi}
• Name: SH3 and PX domains 2A; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7530881
• Synonyms: Sh3pxd2a^fl
• Gene: Sh3pxd2a Location: Chr19:47248613-47452840 bp, - strand Genetic Position: Chr19, 39.45 cM, cytoband D2
• Alliance: Sh3pxd2a^{tm1c(EUCOMM)Wtsi} page
• IMPC: Sh3pxd2a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:340731
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 47266341 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 47267289. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:340731)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sh3pxd2a Mutation: 80 strains or lines available

References

• Original: J:340731 Barbayianni I, et al., SRC and TKS5 mediated podosome formation in fibroblasts promotes extracellular matrix invasion and pulmonary fibrosis. Nat Commun. 2023 Sep 21;14(1):5882
• All: 1 reference(s)