Pf4^tm1.1Ipek
Targeted Allele Detail

Summary

• Symbol: Pf4^tm1.1Ipek
• Name: platelet factor 4; targeted mutation 1.1, Philipp von Hundelshausen
• MGI ID: MGI:7530769
• Synonyms: Cxcl4^L1
• Gene: Pf4 Location: Chr5:90920362-90921242 bp, + strand Genetic Position: Chr5, 44.78 cM
• Alliance: Pf4^tm1.1Ipek page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutation: Insertion
• Mutation details: The targeting vector was designed to insert an FRT-flanked neomycin resistance (neo) cassette and a loxP site upstream of exon 2. A second loxP site was inserted downstream of a mutated exon 3. Exon 3 was altered to contain three amino acid substitutions: proline to leucine (P64L), lysine to glutamate (K72E), and isoleucine to histidine (I73H) to obtain the murine orthologue of human CXCL4L1. Flp-mediated recombination removed the neo cassette. (J:270132)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pf4 Mutation: 18 strains or lines available

References

• Original: J:270132 von Hundelshausen P, et al., Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation. Sci Transl Med. 2017 Apr 05;9(384)
• All: 2 reference(s)